Journal: PLoS ONE
Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells
doi: 10.1371/journal.pone.0058500
Figure Lengend Snippet: a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).
Article Snippet: The luciferase vectors pGL3 (basic promoterless vector containing firefly luciferase), pGL3-pr (bearing an SV40 promoter) and pRL-Null (promoterless vector containing Renilla reniformis luciferase) were purchased from Promega (Madison, WI).
Techniques: Construct, Plasmid Preparation, Luciferase